When facing the fact that high yielding dairy cows has an increasing energy requirements, the practice of adding lipid supplements such as concentrates rich in palm oil and/or palm oil industry by-products become more common. Palm oil containing high levels of C16:0 causes high levels of free fatty acids (FFA) which are linked to larger milk fat globules (MFG) which affect the reaction of lipolysis resulting in rancid flavour and processing problems. Instead of using palm oil, rapeseed oil rich in C18:1 C18:2 can be used. In order to evaluate the effect of added C18:0 and C16:0 on milk lipolysis, 30 Swedish Red lactating dairy cows including 15 multiparous (MP) and 15 primiparous (PP) cows were randomly divided in three groups resulting in 3 10 cow-groups (5 PP and 5 MP cows per group) and fed one out of three concentrate mixtures: control (C), a standard concentrate mixture with no fat added; palmitate (P) as C plus the addition of palmitate methyl ester (10 % in DM basis); and stearate (St) as C plus the addition of stearate methyl ester (10 % in DM basis). Milk yield was registered, and milk samples were collected and analysed for milk composition, FFA content and size of MFG.

Glycerol is essential for the lipid metabolism of both plants and animals. Glycerol is formed in the rumen by hydrolysis of lipids from the feed. Glycerol is also a by-product from the production of biodiesel from rapeseed oil and other fat sources. Glycerol might be used as a dietary glucose precursor for dairy cows in similar ways as propylene glycol. Due to increased production of biodiesel more glycerol has been available to a lower price.

Time of harvest affects nutritional value and digestibility of haylage since plants are in different developmental stages. The aim of this study was to examine the effect of plant maturity of haylage cut in June, July and August on digestibility and faecal particle size in horses.Forage and faecal samples used for the analysis of apparent dry matter digestibility (DMd) and particle size distribution in faeces were from a feed-study performed in the autumn of 2009. Twelve horses were used in the study, divided into three groups in a change-over experiment. Each group was fed haylage harvested in June, July or August during three periods. Faecal grab samples were taken from each horse and pooled so that one sample represented one horse during one period.

Horses are made to eat mainly roughage and it is essential for optimal health to offer them an appropriate amount of roughage with good hygienic and nutritional qualities. To produce good hay at the right time and be able to keep the hygienic quality until next summer is a problem. This has the effect that horse owners today choose haylage and silage to a larger extent. Science in this area is scarce, i.e. if the change from hay to silage has any effect on the horse.

SammanfattningLångvarig smärta orsakar stort lidande för individen och belastar samhället ekonomiskt. Syftet med denna uppsats var att undersöka om en exponering in vivo-behandling inspirerad av Dialektisk beteendeterapi (DBT) för människor med långvarig ländryggssmärta var görbar samt om behandlingen gav effekt på smärtrelaterad problematik. Designen var en single case experimental design vilket är en beprövad design för att testa nya terapimetoder. Studien bestod av sex deltagare, alla med hög grad av smärtrelaterade katastroftankar, något som litteraturen visat predicera sämre behandlingsutfall. Behandlingen var DBT-inspirerad och indelad i tre faser; en första valideringsfas, en andra färdighetsfas och en tredje exponeringsfas.

In the latest edition of NRC (2007) the feeding recommendations for Ca and P to growing horses were increased with 35 and 25 %, respectively, based on results from a Canadian study showing that daily endogenous fecal losses of Ca and P were greater than previously suggested. A Ca absorption efficiency of 50 % is used for all horses. However, the true absorption efficiency might be as high as 70 % in young horses. P absorption efficiency is assumed to be higher for growing horses because their diets are often supplemented with inorganic P. Thus, an efficiency of 45 % is used for growing horses as compared to 35 % for mature horses.

The aim of this study was to examine if the predictions of the herbage quality in the software Grazemore Decision Support System (DSS) gives a reliable ground for milk production in the north of Scandinavia. Pasture samples from one research farm (Umeå) and one organic farm (Nordingrå) was analysed on crude protein and organic matter digestibility. The results were statistically compared to the predicted values. Measured and predicted herbage mass was compared and a control if the predictions of milk production improved if the predicted input were replaced by the values from the analysis, was made. The concentration of crude protein was underestimated by the model on both farms and the relationship between actual and predicted values was poor. Mean Prediction Error (MPE) was 24% and 31% respectively.

The objective of the present study was to evaluate distillers? dried grains with solubles (DDGS) as a protein source to rainbow trout (Oncorhynchus mykiss), by studying digestibility and growth measurements. One control diet, totally lacking DDGS, and three test diets were made containing 10 % (low), 31% (medium) and 51 % (high) DDGS respectively on dry matter (DM) basis. The control diet and the test diets were made to be as nutritionally equal as possible. Wheat bran was used to balance the fiber content of the diets.

Den senast utvecklade tekniken för riktade förändringar av genom in-vivo är baserad på CRISPR/Cas-systemet för vilket förutsättningarna i Nicotiana benthamiana och potatis, Solanum tuberosum, här undersöktes. För att föra in genkonstruktioner i växterna användes Agrobacterium tumefaciens som via vakuum kommer i kontakt med de inre delarna av bladen varefter de gener som finns på en binär vektor uttrycks transient. Först undersöktes de optimala parametrarna för erhållandet av ett högt transient uttryck med hjälp av ett grönt fluorescerande protein, GFP. Dessa parametrar användes sedan för att infiltrera en CRISPR/Cas-konstruktion som designats för att introducera mutationer genom att klyva ett exon i genen för granulbundet stärkelsesyntas, GBSS. Det visade sig under första delen av arbetet att det ej gick att erhålla transient uttryck i potatis med denna metod.

Bakgrund: Ett stifts huvudsakliga uppgift är att vara ett stöd för och bevara den koronala rekonstruktion i en endodontiskt behandlad tand med omfattande förlust av tandkronan. På senare år har efterfrågan för estetiska stift och pelare ökat och nya sorters stift och pelare har dykt upp på marknaden. Zirkonia är ett biokompatibelt och estetiskt material som relativt nyligen har börjat användas som material för stift och pelare. Syfte: Denna litteraturöversikt fokuserar på att analysera både in vivo och in vitro studier som har testat stift i zirkoniumdioxid. Avsikten är att summera senaste årens evidens för materialets relativt nya användningsområde och att försöka besvara frågeställningen om stift i zirkoniumdioxid är att rekommendera för kliniskt bruk. Material och metod: Totalt lästes 361 sammanfattningar och utav dessa sorterades 69 artiklar ut för fulltextläsning. Utav dessa uppfyllde 14 artiklar våra inklusions- och exklusionskriterier.

Destillers dried grains with soluble (DDGS) is a by-product when producing ethanol. DDGS from wheat has a high crude protein content and a good amino acid profile, which makes it an interesting feed ingredient for poultry. The disadvantage of feeding DDGS from wheat to poultry is that it also contains a high fiber content that chickens don?t have the ability to break down. Lantmännen Agroetanol has developed a new protein feedstuff from DDGS by extracting wheat protein from DDGS, the product name is Agroprotein SD.

Tidigt i läkemedelsutvecklingen är det centralt att välja ut substanser som har potential att bli väl fungerande läkemedel. En faktor som tas stor hänsyn till är substansens in vitro potens. Det finns få studier kring hur in vitro potensen förhåller sig till terapeutisk plasmakoncentration av det färdiga läkemedlet hos patienten, men ett vanligt antagande är att den terapeutiska plasmakoncentrationen är en till tre gånger högre än in vitro potensen. I min litteraturstudie har jag fokuserat på följande frågeställningar: - Hur förhåller sig in vitro potens till terapeutisk plasmakoncentration? - Är det sätt som läkemedelsindustrin tolkar in vitro potens till terapeutisk plasmakoncentration optimalt? In vitro potens-data och terapeutisk plasmakoncentration samlades in för nio etablerade läkemedelssubstanser.

AbstractAn approaching overproduction of cereals and a future uncertain supply of non genetic modified vegetable protein feedstuffs have increased the interest in an expansion of the domestic production of protein feedstuffs in Norway. Besides rapeseed (Brassica campestris), peas (Pisum sativum) may be the most suitable crop for this purpose. Peas are characterized by having a relatively high content of crude protein, ranging between 20-26%, a high content of starch, 42-51%, and a low content of fat. In general, the energy value for peas is higher than for barley but lower than for rapeseed and soybean meal.Pea protein consists of albumins and globulins to 85-100%, which leads to the fact that a large part of the pea protein is soluble and degradable in the rumen. Starch in peas is on the other hand to a large extent resistant to rumen degradation compared to starch from other starch rich feedstuffs.

Diabetes mellitus is a rising epidemic throughout the world and there is currently great interest in quantifying the beta-cell mass (BCM) in vivo non-invasively. In the present experiment, the feasibility of in vivo imaging of the glucagon-like peptide-1 receptor (GLP-1R) in beta-cells was examined, using the positron emission tomography (PET) tracer [68Ga]Ga-DO3A-VS-Cys40-exendin-4 as a marker, in native pancreatic beta-cells of a porcine diabetic animal model and healthy controls. Eight Swedish high-health domestic pigs were randomly assigned to be either controls or made diabetic using streptozotocin (STZ). The experiment proceeded during eight weeks, starting with an acclimatisation period. Once the pigs had been socialised they underwent surgery for the insertion of a jugular vein catheter, allowing induction of diabetes with STZ, intravenous (i.v.) injections and stress-free blood sampling. Development of diabetes was confirmed by clinical examinations, blood glucose values and insulin-staining of pancreatic sections post mortem. The diabetic pigs were insulin treated and responded well.

En ny medicinteknisk produkt, ett resorberbart, kirurgiskt buntband (LigaTie®) för ligering av kärl har utvecklats vid Sveriges lantbruksuniversitet. En pilotstudie genomfördes för att undersöka begränsningsområdet med avseende på minsta möjliga kärldiameter som kan ligeras med produkten. Djurmaterialet i detta terminala in vivo-försök utgjordes av en sövd hund. Icke resorberbart band, som formsprutats i samma form som LigaTie®, fördes runt kärl tillhörande krös, mjälte och njurar. Bandets ände fördes genom den låsmekanism som finns i bandets andra ände.